Hepatoprotective and Haemopoeitic Activity of Ethanol Extract of Persea americana Seed in Paracetamol Induced Toxicity in Wistar Albino Rat

The study was designed to evaluate the hepatoprotective and haemopoietic activity of Persea americana. Thrity-five wistar albino rats weighing 100-150g were divided into seven groups consisting of five animals in each. Group A served as normal control, group B served as experimental control while group C-G served as test group administered different doses of Persea americana seed extract. Hepatotoxicity was induced with paracetamol in groups. Experimental period lasted for 14 days after which the animals were sacrificed and blood collected for biochemical estimation. The hepatoprotective effect was evaluated by performing a determining the concentration in serum of alkaline phosphatase, asparate amino transferase, alanine aminotransferase, direct and total bilirubin spectrophotometrically and the haemopoietic effect was evaluated by carrying an assay on the haemoglobin, packed cell volume and total red blood cells by hematocrit method. The results obtained were presented as Mean+SD. ALP in negative control as (788.90±5.80), Normal control (647.76±18.61), the test groups ranged from 610.62±101.28643.14±8.69. AST for negative control is (242.40±1.54), normal control (198.55±11.34), the test groups ranged from 112.35±4.35109.65±2. ALT, Negative control is (86.23±0.37), Normal control (55.09±3.53), the test groups ranged from 46.61±3.30-34.23±1.34, DB, negative control is (8.70±0.17), normal control (7.31±0.14), the test groups ranged from 6.29±0.06-3.35±0.30 and TB for negative control (9.63±0.27), normal control (7.35±0.28), the test groups ranged from 9.11±0.18-4.11±0.22. RBC in negative control as (1050000.00±70710.67), normal control (4300000.00±141421.35), the test groups ranged from 49560000.00±212132.031050000.00±7071.67, HB in negative control as (3.7±0.14), normal control (12.85±0.912), the test groups ranged from 15.50±0.563.15±0.07, PCV for negative control was (10.50±0.70), normal control (37.50±2.12), the test groups ranged from 46.50±2.12-12.50±0.70, Reduction in liver enzymes and increase in haematological parameters in the test group indicated that Persea americana seeds have hepatoprotective and haematological properties. OMODAMIRO O.D 1 , JIMOH M.A and EWA I. C. 3 1&3 Department of Biochemistry, Micheal Okpara University of Agriculture P.M.B Umudike Abia State. 2) Department of plant science and Biotechnology, Micheal Okpara University of Agriculture Umudike Abia